FastQCFastQC Report
Fri 10 Feb 2023
EGAF00001685521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122480823
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG23649011.9308336946756148Illumina Single End PCR Primer 1 (100% over 50bp)
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT1829940.1494062462333389No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG1520870.12417209182208058No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC1410760.11518211303985114No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1372710.11207550426077721No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA1307470.1067489561202573No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA1265920.1033565883207692No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA1229540.10038632741715002No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG1229190.10035775151510862No Hit

[FAIL]Adapter Content

Adapter graph