FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001685604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10321480
Sequences flagged as poor quality0
Sequence length35
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTG322519631.247418005944883TruSeq Adapter, Index 2 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACC143930.13944705604235053TruSeq Adapter, Index 4 (100% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)