FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001685605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001685605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10321480
Sequences flagged as poor quality0
Sequence length35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGA1206771.1691831016482133No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGGAGA1138001.1025550599332654No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGTAGA631730.61205369772552No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGAGA536670.5199545026488449No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGTAGA509450.4935823157144131No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGTAGA491150.4758523002515143No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGA485820.4706883121412821No Hit
GATCGGGAGAGCGGCGTGGAGGGGAAGAGGGGAGA469080.4544697078325976No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGTAGA418330.40530040265543316Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGGCGTGGAGGGGAAGAGGGTAGA406020.3933738184833958No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGTAGA378320.36653658196305183No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGA375290.3636009564519817Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGGAGAGCGGCGTGTAGGGGAAGAGGGGAGA370950.35939613311269314No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGA369520.3580106728879967No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA337620.32710425249092184Illumina Single End PCR Primer 1 (100% over 35bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGA328920.3186752287462651Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGTAGA295730.28651898758705147No Hit
GATCGGGAGAGGGGCGGGTAGGGGAAGAGGGGAGA293030.2839030836662959No Hit
GATCGGGAGAGGGGCGTGGAGGGGAAGAGGGGAGA272600.26410941066591226No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGGAGA269640.261241604886121No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGTAGA268590.26022430891693826No Hit
GATCGGGAGAGCGTCGGGTAGGGGAAGAGGGTAGA260550.2524347283529106No Hit
GATCGGGAGAGCGTCGGGGAGGGGAAGAGGGGAGA245950.238289470114751No Hit
GATCGGAAGAGCGGCGTGGAGGGGAAGAGGGGAGA232330.2250936881144952No Hit
GATCGGAAGAGCGGCGTGGAGGGGAAGAGGGTAGA223710.21674217263415713No Hit
GATCGGAAGAGCGGCGTGTAGGGGAAGAGGGGAGA214410.20773183690711022Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGGGGAGGGGAAGAGGGTAGA214280.20760588597759236No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGTAGA210260.20371109569557855No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGGGGAGA208360.2018702744180098Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGGGTAGGGGAAGAGGGGAGA198750.19255959416672802No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGAGA176310.17081852602533745No Hit
GATCGGAAGAGCGTCGGGTAGGGGAAGAGGGTAGA174770.1693264919372028Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGA172850.16746629359355442Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGGAGAGGGGCGGGTAGGGGAAGAGGGTAGA171930.16657494855388955No Hit
GATCGGGAGAGCGTCGTGGAGGGGAAGAGGGGAGA166610.16142064897669714No Hit
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGGAGA149670.14500827400721603Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGGAGAGGGGCGTGGAGGGGAAGAGGGTAGA149500.14484356894553882No Hit
GATCGGAAGAGCGTCGTGGAGGGGAAGAGGGTAGA143160.1387010389982832Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGGCGGGGAGGGGAAAAGGGGAGA134860.13065955657521983No Hit
GATCGGAAGAGGGGCGGGGAGGGGAAGAGGGTAGA128350.124352321566287No Hit
GATCGGAAGAGCGTCGGGGAGGGGAAGAGGGGAGA127970.12398415731077327No Hit
GATCGGGAGAGGGGCGTGTAGGGGAAGAGGGGAGA125950.12202707363672652No Hit
GATCGGGAGAGGGTCGGGGAGGGGAAGAGGGGAGA122620.11880079213446133No Hit
GATCGGAAGAGCGTCGGGGAGGGGAAGAGGGTAGA121090.11731844657936652No Hit
GATCGGAAGAGCGTCGGGTAGGGGAAGAGGGGAGA120240.11649492127098053Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGGGGCGTGTAGGGGAAGAGGGTAGA111160.10769773327081No Hit
GATCGGAAGAGGGGCGGGTAGGGGAAGAGGGGAGA106720.1033960246011231No Hit
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGA105360.10207838410770549Illumina Single End PCR Primer 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)