FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001698166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13469699
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA5420984.02457397154903No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA4685963.4788899143180556No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2762032.050550647048609No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2422381.7983920798824087Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2240701.663511560280597No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA2218961.647371630204951No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1748311.2979577346160445Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1723231.2793381648691629Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG1648961.224199590503099No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG1420851.0548491098427664Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1278890.9494569997443892Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1209970.8982903032948248Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1176880.8737240527795016No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1077990.8003074159266663No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT995610.7391479200834405Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG953450.7078480372872475No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG917170.6809135081637682No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT831270.6171407393736119No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA763810.567057957271354No Hit
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT763440.5667832666490914No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA749790.5566494099088629No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA717910.532981471969047No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA684770.5083781010993638Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT643270.4775682069807202Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT635100.47150274107832696Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT628870.4668775449250945No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA591260.4389556143756442No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG573080.4254586535304167No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT541450.40197631736239986Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA539140.40026135699097654No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA518180.3847005044433435No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA515820.38294842371756044No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC513890.3815155780392717No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA490460.36412097998626397Illumina RNA PCR Primer (100% over 45bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG466430.34628093768093854Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA449520.33372683383645024No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA447620.3323162603707774No Hit
CGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAG432790.32130636326765727No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA425790.3161095136572836No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC421160.31267216884356513Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG418590.3107641826294708No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA407800.30275361015862345No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN404640.30040760376308334No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA404300.30015518535343666No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC391940.2909790337556912Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA368970.2739259429627937No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG359090.266590960941295No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT356120.26438601189232214Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA350610.26029534884187094No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG350460.2601839877787915No Hit
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT337430.2505104234326246Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGAC331740.24628612710647801No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG331190.2458778032085201Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTG329200.2444004131049996No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG326040.24205440670945952No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA321850.2389437210141073No Hit
TGTTAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGA319000.236827860815598No Hit
GGTGCATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTG306400.2274735315169255No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC306390.22746610744605353Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA302200.22435542175070133No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG300660.22321211483641912Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT291480.2163968177759577Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT284070.21089558125983363Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG283920.2107842201967542No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC278730.20693112741420575Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT277350.20590660563387497No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTG277170.20577297235817965Illumina DpnII expression Sequencing Primer (100% over 23bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC275020.20417679712070774No Hit
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA271600.20163776488249663No Hit
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACT271100.20126656133889853No Hit
GCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAGA264920.19667848554002582No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT260310.19325598886805118Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA250900.18626993817753462No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT240530.17857117668330968Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG236280.17541594656272572Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG235830.1750818633734874Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTA235470.17481459682209677No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT222160.16493315849151494Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG214450.15920919984923199Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT211480.15700425080025915Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG208970.15514080901139662Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC202130.1500627445349744Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA199870.14838490451791092No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGATCGTCGGACTGTAGAACTCTGAA197600.14669964042997546Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA194690.14453923580623443No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA192890.1432029030492812No Hit
CATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTGTAGA191800.1423936793242373No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG188610.14002540071608133No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT187440.13915678442406174Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT181980.1351032417279703Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT180920.1343162902155423Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGAA173440.12876308520331448No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG168470.12507332197994922Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCTAGTCAAGTGAAGCAGTGGGAGATCGTCGGACTGTAGAACTCTGAA164850.12238580832429886Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC161780.12010661856660643Illumina DpnII expression Sequencing Primer (100% over 26bp)
TATCAGATATTTTATTGTCATCAAGTAAGATCGTCGGACTGTAGAACTCT160830.11940133183377Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGGATCGTCGGACTGT160200.11893361536883637No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG155480.11542945391727016Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAGATCGTCGGACTGT155270.11527354842895896No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT155130.11516961143675149Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG150610.11181393140262451No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG150450.1116951462686731Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAG149010.11062608006311055No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC147910.10980943226719468No Hit
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG147000.1091338418178461Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC146730.10893339190430314Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCCAGTCAAATTTAGCAGTGGGGATCGTCGGACTGTAGAACTCTGAAC144300.10712934268241629Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAAC136930.10165780244978007No Hit
AGGCTAGTCAAGTGAAGCAGTGGGAGGATCGTCGGACTGTAGAACTCTGA136640.10144250439449315Illumina DpnII expression Sequencing Primer (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)