FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8552489
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3066713.58575146954296Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG2876233.363032679726335No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2764403.2322754229792054Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2424652.8350226466236905No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2174612.542663311230216Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1910982.234413864782521Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1329161.5541206776179426No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1305171.5260703638437887Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1293341.5122381332498644No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1231921.4404227821865658No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1032131.206818272435077Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG835760.977212598578028Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT717040.8383992075289427Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC716450.8377093498746389No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA673200.7871392760633776No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA611330.7147977623823896No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG601800.7036548073899891No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC568620.6648590837123556No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG520230.6082790635568196No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG517920.6055780954526805Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT497770.5820177026828096Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA478200.5591354750646274No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG473760.553944003903425Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG450420.5266537027992669Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC444660.5199188212928424Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA420790.4920088175500723No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG406990.47587316394092993Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA404950.4734878934074045No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC355880.41611278307402677Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT348800.40783449122237986Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG340290.39788417149674205No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC336410.39334748048199775Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG326580.3818537504111376Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT318040.3718683531776539Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT314270.36746027969167805Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT304260.35575608457374225Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT295020.3449522121571861Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG288930.33783147806445585Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC283450.3314239866312602No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG282220.3299858088095758No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA278530.3256712753445225No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT270080.31579111063457665Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG268930.3144464728338148Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA257810.3014444099255784No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN254220.2972468014866783No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG247470.2893543622213369Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT244610.2860103064733553Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA220560.2578898376835094No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG213510.24964662333970847Illumina DpnII expression Sequencing Primer (100% over 23bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT207780.2429468193411298Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT206300.2412163289540624Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA204530.23914675599115065No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG204340.23892459844145958No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG201920.23609501280855197Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG199120.23282111207626222Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG197640.2310906216891948Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT193770.22656562317706577No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG191350.2237360375441582Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC189740.22185354462309162Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA187660.2194215040791049No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT183110.21410141538913408No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT181230.21190322489745383No Hit
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC177780.20786931149516827Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC169210.19784883675383858Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT163740.19145303782325823Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC163100.1907047176558777Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT161120.18838960213804426No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA156490.18297597342715086No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT154170.18026331282039648Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA150960.1765100194808786No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT149700.17503676415134822Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG147550.1725228760890543Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA146340.17110808327260055No Hit
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT144570.1690385103096888Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA144570.1690385103096888No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTA142860.167039092362469No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG140950.16480582436294275Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG139000.16252578635295528Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT136860.16002359079327666Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG135820.15880757052128333Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT131250.15346409682608186Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT128300.1500148085545623Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG127310.14885725079564557Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC123980.14496364742474385No Hit
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT123390.14427378977043992No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT122520.14325654204290705Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG121120.14161959167676216Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT120490.140882964011997Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA120000.14031003138384626No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC119710.13997094880800198No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA117290.1371413631750944No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA115940.13556287532202613No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA114920.13437024005526343No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA114160.13348160985649907No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG113720.132967139741425Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA113570.1327917522021952No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT113120.13226558958450574Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT112940.13205512453742999Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAG111860.13079233425497536No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA111410.13026617163728593No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA108550.12692211588930427No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT107590.12579963563823351Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT105890.12381191019362901Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT105780.12368329266486049Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT105770.12367160016224517Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG103030.12046785444564734Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT99360.1161767059858247No Hit
GGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAAC98940.11568562087598125No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT98580.11526469078182972No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG98260.11489053069813945No Hit
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT97760.11430590556737344Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT97620.11414221053075894Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT96740.11311327030061073Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG95600.1117803250024642No Hit
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT93820.10969905953693714Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA92110.10769964158971733No Hit
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT91510.1069980914327981Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT90520.10584053367388135Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT89870.10508052100388554Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG86910.10161954022975066No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG86390.10101153009375399No Hit
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT85930.10047367497344926Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)