FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9299606
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4836665.200930017895382Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2729672.935253386003665Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG2581032.77541865752162Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2110402.269343453905466Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2070532.226470669832679Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1887022.0291397291455144Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1785631.9201136048129352No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1562741.6804367840960142No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1187771.2772261534520923Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1026551.1038639701509934Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG707510.76079567241881No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA705920.7590859225648915No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC628030.6753296860103536Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG618400.6649744085932243Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA584770.6288115862112867No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG577600.6211015821530503No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG504030.5419907036921778Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT468230.5034944491196723Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG452180.48623565342445685Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT451230.4852141047695999Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT448790.4825903376981778Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC444470.47794497960451227Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC443670.4770847281056853Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG440840.47404158842858507Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT434500.4672240953003815Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG425220.45724517791398905Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT411790.4428037058774318Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA405820.43638407906743576No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT394840.424577127246036Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC393840.4235018128725024Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT388250.4174908055244491Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT386280.41537243620858777Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC365590.393124181820176Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT364450.3918983234343476Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT347080.3732201127660677Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG346650.3727577275854483No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG340180.36580044358868535Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT327330.35198265388877764Illumina DpnII expression Sequencing Primer (96% over 28bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC320360.344487712705248No Hit
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT301340.32403523332063744Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT287300.3089378195162247Illumina DpnII expression Sequencing Primer (96% over 28bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN279250.3002815388092786No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG266140.2861841673722521Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT262160.2819044161655881Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG257880.27730207064686396Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG249230.26800060131579767Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT249030.267785538441091Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT243500.2618390499554497Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG234370.25202142972508723Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA231220.2486341894484562No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA227450.24458025426023425No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC222740.23951552356089065No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT221110.23776276113203076Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG220020.23659066846487906Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA217070.2334184910629547Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG216530.23283782130124653Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT215960.2322248921083323Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG215130.23133238117829935Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT213060.22910648042508466Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC208780.22450413490636054Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT196130.21090140808115954No Hit
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC195150.20984759999509656Illumina RNA PCR Primer (100% over 32bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT194300.20893358277759294Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT192350.20683671974920229Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA190370.2047075972896056No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT186720.2007826998262077Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT183070.19685780236280975Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA182690.196449182900867No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG179690.19322323978026595Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG177030.19036290354666638Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA173190.18623369635229708No Hit
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG172760.1857713111716776Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT172670.18567453287805954Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG168020.18067432104112796Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG160230.17229762207130064Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG157070.16889962865093425Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCTTTTAACATTGCACTGGATCGTCGGACTGTAGAACTCTGAACGTGT154150.16575971068021592Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG148650.15984548162578072Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG147400.1585013386588636Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT146850.1579099157534201Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT146620.15766259344750733Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA145850.15683460137988642No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG145630.15659803221770902Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT143210.15399577143375753Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA141800.15247957816707502No Hit
TATCAGATATTTTATTGTCATCAAGTAAGATCGTCGGACTGTAGAACTCT133500.14355446886674553Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG131880.141812459581621No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA130510.14033927888987985No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA130010.13980162170311303No Hit
GCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG127820.13744668322507428Illumina DpnII expression Sequencing Primer (96% over 26bp)
GATCACTTTTGTGACTATGCAAGATCGTCGGACTGTAGAACTCTGAACGT127430.13702731061939613Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT123940.1332744634557636Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG122670.13190881420137585No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG120980.13009153291010392Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA120880.12998400147275058Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA120580.12966140716069047No Hit
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGAACTCTGAACGTG120330.12939257856730704Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCTAGTCAAGTGAAGCAGTGGGAGGATCGTCGGACTGTAGAACTCTGA118180.12708065266420965Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG116980.12579027541596924No Hit
AAACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG115560.12426332900555143Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCTAGTCAAGTGAAGCAGTGGGAGATCGTCGGACTGTAGAACTCTGAA114240.12284391403248696Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACGTG111000.11935989546223787Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTACAATCAACGGTCGATGGTGATCGTCGGACTGTAGAACTCTGAACGT108850.11704796955914047Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT108510.11668236267213901Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA106140.1141338676068642Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGTA105700.11366072928250938Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG102170.10986486954393553No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA99830.10734863390986671No Hit
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC98470.10588620636186091Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA98360.10576792178077221No Hit
TAACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG97650.10500444857556332Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT97630.10498294228809264Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA96270.10352051474008683No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC96020.10325168614670342Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT94610.10173549288002093No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA94500.10161720829893223No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA94130.10121934198072477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)