FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12394735
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT8422396.795135192482937Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG6555275.288753652256382Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4502473.6325665695958813Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3300892.6631388246703134Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2886772.329029220874831No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2540192.0494104956661032No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2064791.6658605448200388Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1972161.5911272003798387Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1690301.3637241941840628Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG1660961.3400528530864113No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1448551.1686817023518454No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1271001.025435396561524No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1093160.8819551204604212Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT994090.8020260215325297Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG910010.7341907672894984Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG885680.714561465009135Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA855720.6903899115229168No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA843570.6805873622953618No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG742120.5987380932307145No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC734200.5923482833638638No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG692700.558866325096906Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG690730.5572769405719445Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG669850.5404310781957017Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT644650.5200998649829948Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG641020.5171712021273549No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT585700.47253934836041267Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC581450.4691104731162062Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG563050.45426546029422976Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA536740.4330387055471537No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA534330.4310943315851449No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC513190.4140387027233741No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT497800.40162214036847094Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC459310.3705686325685866Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT455850.3677771247227149Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC440830.35565907621260157Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC439110.3542713902314168No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT436070.3518187359390903Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG419770.3386679908848394Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT415930.33556990125242697Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA384700.31037371916382234No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN375960.3033223380733836No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT374120.3018378367911859Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA370940.2992722313143444No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC368440.2972552458765758Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG365670.2950204260115283Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT361640.2917690454858454Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT359110.28972785622282365Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG351120.2832815707637154Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG335610.2707681931077994Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG327060.263870102910631Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA306510.24729048261217362No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT303900.2451847498151433Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG303860.245152478048139Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG302760.24426500445552082Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA289560.23361532134410298No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG283960.22909727396350144No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA282220.22769345209881453No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG276740.2232722200192259Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT273510.22066627483362894Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC268510.21663230395809188Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA267820.21607561597726777Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG258080.20821744071172155Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT257950.2081125574689576No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG255030.20575671847764396Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT255020.20574865053589286Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA252430.20365905362236467No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT250890.20241659059269923Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT249870.20159366053408967Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA249490.20128707874754884No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA237750.19181531513178782No Hit
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG236130.19050830856811382Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA225990.1823274156325246No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT223680.1804637210880265Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT223140.1800280522334685Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT221030.17832571652399185Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG214130.17275883671575068Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC211920.1709758215887633Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT210510.16983824180186186Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA209710.16919280646177592No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT208780.168442487878926Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT207600.16749047075229928Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG198350.16002762463255568Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT197540.15937412135071866Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG197320.15919662663219503Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT191580.1545656280670785Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC191390.15441233717380806Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT187150.15099152987135261Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA176140.14210872600342No Hit
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC175820.14185055186738563Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT170430.13750193126355667Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC168260.13575118790357357Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT165970.1339036292425776Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT165460.1334921642132728Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG165230.13330660155299812Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT164920.1330564953587148No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG163400.13183016821255153No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT163330.131773692620294Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG161920.13063611283339258Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA159200.1284416326771004No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG157800.12731212083195Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA154870.12494821389888529No Hit
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT150730.12160808601394059Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT137160.11065988905773298Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT134040.10814269123139784Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA126880.10236604493762876Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT124180.10018770066483873Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)