FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001698174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840597
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1387664.885099857529949No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1285524.525527556355231No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA1226224.316768622933841No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1040553.663138417734019No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG962413.388055398213826No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA503521.7725851291119439No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA349361.2298823099510419No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG339341.1946080348602777No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC332921.172007152017692No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT320351.1277558907511345Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA279910.9853914511632591No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC277020.97521753349736No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA273520.9628961799227416No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG238820.8407387601972403Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA199100.7009089990590006No Hit
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC183450.6458149466467789No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA162690.5727317180156144No Hit
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA137920.48553173857467286No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT130970.46106505076221654Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA130540.45955128446590626No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA126800.4463850380747428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120430.4239601745689375No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG116890.41149800552489496Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT113380.39914144808292057No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG112640.3965363618985728No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT112520.39611391549030006No Hit
CGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAG111220.3915374127340133No Hit
GCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAGA111200.3914670049993012No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA109450.38530632821199207No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG108320.3813282912007581No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA106550.3750972066787369No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT106200.37386507132127506No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA102630.3612972906751644Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA101870.35862179675610445No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT101830.3584809812866802No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG98350.34623003544677405Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG96970.34137190175163884No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA96920.3411958824148586No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG96030.3380627382201699No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA93000.3273959664112861No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA92590.32595260784968794No Hit
CATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTGTAGA88550.31173024543784283No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC86520.3045838603645642No Hit
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA84850.2987048145161035No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA82980.2921216913205217No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGATCGTCGGACTGTAGAACT78200.27529424272432873No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT77870.274132515101579Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA76290.26857030405932275No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG75050.26420502450717226No Hit
AGGCGAACGTGATAACCACTACACTACGGAAAGATCGTCGGACTGTAGAA74500.2622688118025894No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC71460.2515668361263495Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT70670.24878573060522136Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAGATCGTCGGACTGTAGAAC67520.2376965123880649No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT60390.2125961549631996Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCCGATAGCTTATTTAGCTGACCTTACTGATCGTCGGACTGTAGAAC56490.19886664669433923No Hit
CGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACTCT53970.1899952721206141Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT53400.18798865168131912Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG52930.18633406991558465No Hit
GGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAG52050.18323612958825206No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC51830.18246164450641889No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA50990.1795045196485105No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG50490.17774432628070788No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA49720.17503362849429188No Hit
GTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAAC49230.1733086389938453No Hit
TGGGCCCGATAGCTTATTTAGCTGACCTTACTGATCGTCGGACTGTAGAA49000.1724989500446561No Hit
GGTGCATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTG48630.17119640695248217No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA48390.1703515141359369No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA48360.17024590253386876No Hit
TGGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTA47090.16577501137965012No Hit
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG45980.16186738210312832Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC44710.15739649094890967Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT44660.15722047161212943No Hit
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG44570.15690363680592495No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT43980.15482660863191788Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCTGACCGGGAAACGAACCCGGGCCGCGGCGGATCGTCGGACTGTAGAAC43110.15176387217194132No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGATCGTCGGACTGTAGAACTCTGAA42210.14859552410989663Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGAA41830.14725777715036664No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT40940.14412463295567798Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC40360.14208280864902695Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG40180.14144913903661802Illumina DpnII expression Sequencing Primer (95% over 24bp)
AAGCCAGTCAAATTTAGCAGTGGGGATCGTCGGACTGTAGAACTCTGAAC40020.14088587715892115Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA39860.14032261528122433No Hit
TATTCTCGCACGGACTACAACCACGACCAGATCGTCGGACTGTAGAACTC39360.13856242191342172Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATTGGCCGGGAAACGAACCCGGGCCTCCCGCGTGATCGTCGGACTGTAG39190.13796395616836882No Hit
TGAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA35940.12652269927765186No Hit
GCCGCCCGGGAAACGAACCCGGGTCGCAAGAGATCGTCGGACTGTAGAAC35540.12511454458340976No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG34250.12057324569447901No Hit
GGTGTTTATGGGGTGATGTGAGCCCGTCTAAGATCGTCGGACTGTAGAAC33700.11863703298989614No Hit
GGCAGGCGAGAATTCTACCACTGAACCACCAATGATCGTCGGACTGTAGA33640.11842580978575983No Hit
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAGATCGTCGGACTGT33420.11765132470392668No Hit
TAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTG33200.11687683962209354No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC32910.11585592746876801No Hit
GAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACTCT32530.11451818050923802Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACGCAGAGTACTAACCACTATACGATCACGGATCGTCGGACTGTAGAAC32250.11353247222326857No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG31560.11110340537570096Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC31170.10973045454881492Illumina DpnII expression Sequencing Primer (100% over 21bp)
TATTCTCGCACGGACTACAACCACGACCAATGATATGAAAAACCATCGTT30870.10867433852813335No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT30830.10853352305870913Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTG30180.10624527168056573Illumina DpnII expression Sequencing Primer (100% over 23bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA30030.10571721367022496No Hit
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACT29550.10402742803713445No Hit
TAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG28890.101703972791635Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT28800.10138713798543053Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)