FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001698176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126369
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG2125015.149830274510108No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1431373.4688366454866255No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1216122.94719158659829No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1088452.6377912397073553No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1047812.5393027138387283No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA900672.182718026429532No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG863432.0924691902251107Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT666571.615391158667584Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG651141.5779975082209081Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT603751.4631507749306958Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC584781.4171781534806993No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG566221.3721991416666808No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA551271.33596874152554No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT412801.000395262760068Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT385970.9353744175569368Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA365440.8856212326139519No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG353130.8557887091532532Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA337510.8179346054606361No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA291390.7061656386038185No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC287830.6975381988377675No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA272540.6604838297302059No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC245350.5945905467979233No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT243780.5907857489235694Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA230620.5588933030468192No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG226490.5488845035429454Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT221210.5360887501820608Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA205220.49733797437892735No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT204470.495520395776529Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA193900.4699046546733945No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC190120.4607440585173066No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA178880.4335046138626963No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173310.4200060634422176No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA160560.38910722720144514No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG159820.3873138829804121No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC156820.38004356857081856Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA153800.3727247853984944No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG150340.36433968944609657No Hit
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA146040.3539189054590125No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG145870.3535069209758022Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC142990.34652741914259244No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG139860.3389420577752499No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT138370.33533113495181843Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT132920.32212339710772353Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA124390.3014514698031126No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC121370.29413268663078845Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA120540.2921212329774676No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG115410.27968899533706265No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG114820.2782591668365093Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA112570.2728064310293142No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT111300.26972866459591954No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA111270.2696559614518236No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC110160.266965945120274Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT109520.26541494471289406Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG109330.26495449146695316Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT104110.2523041443942604Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT102380.2481115964180615Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG97920.23730306232913248Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT94340.22862715380035087Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT93780.2272700284438934Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGA93590.2268095751979525No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT92820.22494352783282348Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG88630.21478932204075785Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG88570.214643915752566Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT88510.2144985094643741Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT88390.2142076968879904Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAG85390.20693738247839688No Hit
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC84070.2037384441381757No Hit
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG82690.20039409950976272Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT81960.19862498967009495Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC81660.19789795822913558Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA81540.19760714565275184No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG77240.1871863616656678Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT76760.18602311136013283Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA74710.18105506318024392No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT74390.18027956297655398No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG73740.17870432818780868Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG73530.17819540617913715Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG73220.1774441403568125Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA71840.17409979572839948Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT70190.17010112280312303Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC69380.1681381379125328Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA69340.16804120038707154No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT69290.16792002848024498No Hit
AGCGCCGAATCCTAGCCACTAGACCACCAGGGAGATCGTCGGACTGTAGA66900.16212801133393548No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT64220.15563319712803195Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG62720.15199803992323518Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA60770.14727233555699937No Hit
GAGCGCCGAATCCTAACCACGAGACCACCAGGGAGATCGTCGGACTGTAG60680.14705422612471158No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG59190.14344330330128013Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG57350.13898417713006278Illumina RNA PCR Primer (100% over 36bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC56990.13811173940091157Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT55890.13544595745072727Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG54240.13144728452545082No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT54200.13135034699998957Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT54120.1311564719490671Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT53550.12977511221124433Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTA53350.12929042458393808No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA52790.12793329922748062Illumina RNA PCR Primer (100% over 45bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA52000.126018783099621No Hit
TGGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT50580.12257750094574672Illumina RNA PCR Primer (100% over 35bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA49720.12049334414832992No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG48380.11724593704537817Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT47470.11504060834113478Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC46620.1129806859250833Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG46150.11184167000091363Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGGGATCGTCGGACTGTAGA45800.11099346665312773No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCCGATCGTCGGACTGTAG45630.11058148216991744No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG45370.10995138825441932Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA44800.10857002851659654No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT44580.10803687212655969Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC44170.10704326249058192Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGATCGTCGGACTGTAGAACTC44120.10692209058375535Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCCTGACCGGGAAACGAACCCGATCGTCGGACTGTAGAACTCTGAACG42690.10345657404851578Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGAGAGCTTGCCCTTGGATAGATCGTCGGACTGTAGAACTCTGAACGT42690.10345657404851578Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAA41790.10127547972563773No Hit
GAGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAG41320.10013646380146807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)