FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001698180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725325
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA978042.625381678108621No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT904462.427868709441458Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA833092.2362881090911535No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA818382.1968016213350516No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG770852.0692154375792717No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT676921.81707636246502Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA627521.6844704824411292No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG433561.163817921926275Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG413321.1094870917302517No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT310670.8339406628951835Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA281240.7549408440874287No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC272460.7313724305933039No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT265000.7113473321119634Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT250560.6725856133357492Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG232070.6229523598612201Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT230380.6184158429130344No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA227530.6107655036808869No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG226470.6079201143524391Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG225620.6056384342305705Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA224220.6018803728533753No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA221840.5954916685121432No Hit
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC211470.5676551710253468No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT211280.5671451484098703Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG202180.542717749458101Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT193380.5190956493728736Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC179360.48146134900981796No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT174560.46857656714514845Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA172830.4639326770147571No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA171670.4608188547307953No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG166530.4470214008173784Illumina DpnII expression Sequencing Primer (95% over 24bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG160930.4319891553085972No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC157710.42334561414104804Illumina DpnII expression Sequencing Primer (100% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157540.42288927811667437No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA154050.4135209679692376No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG144610.3881808969687209No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA124840.33511170166361326No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG110700.2971552817539409No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT110150.2956789004986142No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG108230.29052498775274643No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT107290.2880017179709153Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT105860.28416312670706584Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC105210.2824183124962252No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA104530.28059296839873027No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT102200.2743384805352553Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA101550.2725936663244147No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT100710.27033882949809745No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA100010.2684597988094999Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGTGCATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTG98230.26368169220135157No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG97430.26153422855723996No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG95410.25611188285585823Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC93550.25111902988329876Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC91940.2467972592995242Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG89780.2409991074604229Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTG89480.2401938085938811No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA86300.23165764060853752No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA84720.22741639991141713No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT84310.22631582479380993No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT81110.21772597021736362No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA80960.21732332078409264No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG80800.21689382805527035Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAG79670.21386053565796273No Hit
TGGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTA79160.2124915275848416No Hit
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA75610.20296215766409642Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACTGTAGA74200.19917725299134972No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT74010.19866723037587328Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG73300.19676135639172423No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT73010.19598290082073377Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT71400.1916611302369592Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA70340.18881574090851133No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG70070.18809097192862367No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT69270.1859435082845121Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT68650.18427922396032562Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG68280.183286022024924Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG68170.18299074577385865Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA67810.18202438713400843No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA67300.1806553790608873No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT66300.17797104950574782Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG62050.166562648896405Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC61440.16492520786776993Illumina DpnII expression Sequencing Primer (100% over 26bp)
CATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTGTAGA60390.16210666183487346No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA60070.16124767637722884No Hit
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAGATCGTCGGACTGT59960.1609524001261635No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA58630.15738224181782798No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT58550.15716749545341682Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTGCGCCGCCCGGGAAACGAACCCGGGTCGCAAGAATGATCGTCGGACT58430.1568453759068001No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT58410.15679168931569729Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG57730.15496634521820243Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG55790.14975874588123184Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG55740.14962452940347487Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTGAACG55080.1478528718970828Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAG54620.14661808030171863No Hit
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA53960.14484642279532658No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT53380.14328951165334566Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA52030.13966566675390737No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT51480.13818928549858064Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTGTTTATGGGGTGATGTGAGCCCGTCTAAGATCGTCGGACTGTAGAAC50610.13585391878560932No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG49380.13255219343278776No Hit
GGGCCCGATAGCTTATTTAGCTGACCTTACTGATCGTCGGACTGTAGAAC48840.13110265547301242No Hit
TATTCTCGCACGGACTACAACCACGACCAATGATATGAAAAACCATCGTT47890.1285525423956299No Hit
TGAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA47390.12721037761806017No Hit
TATTCTCGCACGGACTACAACCACGACCAGATCGTCGGACTGTAGAACTC47160.1265929818203781Illumina DpnII expression Sequencing Primer (100% over 21bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT46270.12420392851630396Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC46120.12380127908303301Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG45490.12211015146329515Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAACTCT45490.12211015146329515Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGGCCCGATAGCTTATTTAGCTGACCTTACTGATCGTCGGACTGTAGAA45470.12205646487219235No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG45320.12165381543892144Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCAGTCAAATTTAGCAGTGGGGGATCGTCGGACTGTAGAACTCTGAA44160.11853999315495964Illumina DpnII expression Sequencing Primer (100% over 25bp)
TAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG44080.11832524679054848Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGCCAGTCAAATTTAGCAGTGGGGATCGTCGGACTGTAGAACTCTGAAC42390.11378872984236275Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG39590.10627260708797219Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT39340.10560152469918731Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA37960.10189714991309484No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA37740.10130659741096415No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTG37740.10130659741096415Illumina DpnII expression Sequencing Primer (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)