FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001698182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254285
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG1849985.684751028259663No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1809795.561252318097524No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1599954.916440938639363No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1359824.178552277996549No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1065623.2745134491908363No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1003423.0833808348070315No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT814162.501809153162676Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC742442.281422801014662No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG664202.0410013259441016Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG578741.7783937178212725No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC515771.5848949922947744No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT444391.3655534164954821Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT409591.2586174843321958Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA378461.1629589909918767No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG368771.1331828650533067No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA339181.0422565939983746No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC325921.0015103163982257No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA302470.9294514770525629No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT283910.8724189798988103Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT259670.7979325719781765Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG200780.6169711626363394No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA191420.5882090843303521No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA165700.5091748264211647No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT158560.48723452309800774Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA158470.48695796465275787No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG154030.4733144146870972Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA148450.45616779108160466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137010.42101413981873126No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA128410.3945874439392985No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT123450.3793460007344163No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC122030.374982523042696Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA120840.37132580582216984No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG118740.364872775433006Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA117330.3605400264574246No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT115030.35347242174548327Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA113880.3499386193895126No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG102620.31533808501713895Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA101290.3112511657706685No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG101060.31054440529947436Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT95140.2923530053452602Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA86730.266510155072466No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG85850.26380602805224496Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC84870.2607946138706352Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA82610.25384992402324935No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA79840.24533806965278088No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAG78910.24248029905186547No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT77750.23891576797975592Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG77340.23765589061806205Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG74730.22963569570581557Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT73200.22493420213656762No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC70810.21759003897937643No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC69290.21291927412626738Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG68260.20975421636396321No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA66590.20462252076877105No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG66410.20406940387827127No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG64100.19697107045019108Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG63730.19583410795305267Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT61260.18824411506675046Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT60490.18587800392405704Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAG59330.18231347285194752No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT58940.18111505292253136No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG58150.1786874843475602Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACGAGACCACCAGGGAGATCGTCGGACTGTAG58140.17865675563142133No Hit
AGTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGA55250.16977615666728638No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG54690.16805534856350934No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT53350.16393770060090004Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT52730.16203252020028977Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC51790.15914402088323548No Hit
GAGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAA50920.15647062257915334No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG50900.15640916514687558Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT48710.14967957631246187Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT48340.1485426138153235Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA47820.14694472057610197No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT45250.139047440528411Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT43730.13437667567530195Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG42790.13148817635824767Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA42460.13047412872566477No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG42240.1297980969706095No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT41960.128937692918721Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG41640.127954374002277Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA41590.12780073042158263No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA40990.1259570074532501No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGGGATCGTCGGACTGTAGA40570.12466640137541733No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA40540.12457421522700071No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT40170.12343725272986232Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT39950.12276122097480706Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC39270.12067166827736354Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG39140.12027219496755816No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG38520.11836701456694788Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGTGCCCCGTGTGAGGAACGAACTCACGACCTTCAGAGATCGTCGGACT38410.11802899868942025No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG38140.11719932335367061Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA38100.11707640848911512No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG37860.1163389193017821Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA37680.11578580241128236No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG37420.11498685579167159Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT37190.11428009532047746Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA37020.11375770714611658No Hit
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA36970.1136040635654222No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC36300.11154523958411756Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT35960.1105004632353958Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT35680.10964005918350729Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT35530.10917912844142415Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT35360.10865674026706328No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG35330.10856455411864664Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT34730.10672083115031412No Hit
GAGCGCCGAATCCTAACCACGAGACCACAGGGAGATCGTCGGACTGTAGA34370.10561459736931461No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA34230.10518439534337035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)