FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7269723
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3540344.8699792275441585Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA3413304.695227039599721No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3060204.2095138975721635Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2833603.8978101366448215Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1937522.6651909570694783Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1795742.4701628934142335Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1334911.8362597859643344Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1061971.460812193256882Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1016041.3976323444510883Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1003821.3808229006799848Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA851861.1717915524429197No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG812731.1179655676014066Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC694590.955455936904336Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT632890.8705833771107921Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG556280.7652010950073338Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG520590.7161070648771625Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC486780.6695991030194686Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG460560.6335317040277876Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT425300.585029168236534Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC405390.5576416047764131Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT384830.5293599219667654Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT384150.5284245355703374Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA381300.52450416611472No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG349250.4804172043418986Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT346280.47633176669867616Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT335420.4613930957204284Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG331000.4553130841436462Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG316290.4350784754797397Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG294960.40573760513296037Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT293820.40416945735071336Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA271430.37337048468009026No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA265420.3651033196175425No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT261980.3603713649062007Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC261850.36019254103629533Illumina DpnII expression Sequencing Primer (100% over 26bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT257750.3545527112931263Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT253490.3486927906331507Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT246620.33924263689276746Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA245530.3377432675220225No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT241610.3323510400602609Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG241160.33173203435674237Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG236310.3250605284410424Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT233370.32101635784472116Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG233150.32071373283411214Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT226630.3117450279742433Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG225810.31061706202560946Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT219640.3021298060462551Illumina DpnII expression Sequencing Primer (96% over 28bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN219160.30146953329583537No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT193880.26669516844039315Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG188680.25954221364417873Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA186590.25666727604339257No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC185250.2548240146151373No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG175480.24138471300763453Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT171530.2359512184989717Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC171060.2353047014308523Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG170590.23465818436273295Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG166190.22860568415055152Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG164930.22687246818069962Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT163930.22549689995065839Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG159700.21967824633758398Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT158670.21826141106064154Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC157540.2167070189606949No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG156510.21529018368375247No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC154850.213006740421884Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC151340.20817849593443932Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC149130.2051384901460482Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG148620.20443695034872716No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT147110.20235984232136492Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT144770.19914101266306847Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG143420.19728399555251278Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG142290.1957296034525662Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT140480.1932398249561916Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG137700.18941574527667698Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT133230.18326695528839268Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA131860.1813824268132362No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT131380.18072215406281644No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA131270.18057084155751188No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT130510.17952540970268055Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG129240.1777784380505282Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG128350.1765541823257915Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT123580.16999272186849484Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA123070.16929118207117383No Hit
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT123010.16920864797737137Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA122030.16786059111193094No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA120690.1660173296836757No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT120060.16515072169874973Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT117890.16216573863956027Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG115920.15945586922637905Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG115650.15908446580426794Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT111440.15329332355579436No Hit
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT109960.15125748257533334Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT106130.14598905625427544Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG105990.14579647670206966Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT103280.14206868679865792Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG103180.1419311299756538Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT102050.14037673787570723Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA99610.13702035139440663No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA99410.1367452377483984Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT98080.13491573200244356Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT97300.1338427887830114Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG96690.13300369216268623Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG96460.13268731146977678Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT96270.13242595350606895Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT95160.13089907277072318Illumina DpnII expression Sequencing Primer (96% over 28bp)
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG91470.12582322600187104Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC89420.12300331113028654Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC89220.12272819748427828No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC89160.12264566339047582Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA88870.12224674860376386No Hit
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT87820.12080240196222056Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC86670.11922049849767316Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG82530.11352564602530248Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG80630.11091206638822414Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT79880.10988039021569322Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG79430.10926138451217468Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAAC78100.10743187876621983No Hit
AAACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG77560.10668907192199757Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG77500.10660653782819511Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG76210.10483205481144192Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG74640.1026724126902772Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG74540.10253485586727307Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACG74480.10245232177347059Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATGCAACCTACTACCTCTGATCGTCGGACTGTAGAACTCTGAACGT73940.10170951492924833Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT73740.10143440128324009Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)