FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601587
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA238390318.91748237741802No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA9573247.596852682126465No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA9417977.473638042573527No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC6489525.149764073366315No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG2802362.2238151432831437No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2372151.882421634671887No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1759981.3966336144804619No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA1522931.2085223869025385No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1519051.2054434096276923Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1473701.1694558788508147No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1449241.1500456252057776No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1369011.0863790409890437Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1308841.0386310867036033No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1291601.0249502701524815No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA1244900.9878914457361601No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1236890.9815351034754591No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1181850.9378580650199059No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1068130.8476154630365208No Hit
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT1055770.837807174604278No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT968330.7684190888020691Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA822650.6528146018434027No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG811710.6441331556096863No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA774570.6146606772623162No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG731560.580530055460475No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT711330.5644765218856959Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA617930.4903588730530528No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT605750.48069342377273594Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA591020.469004419840136No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC576470.4574582550594619Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA559790.44422182698099844No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT502580.3988227831938945Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA470060.373016509745955No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC458450.3638033844467367No Hit
AATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGAA392210.3112385765380186No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN390120.30958005527399046No Hit
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA377700.29972415379110584No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT353930.2808614502284514No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA336450.2669901814747619No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG323690.25686447270490614Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT307020.24363598013488302Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC253020.20078423455712363No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT228740.18151682006401257No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG224700.17831087465412096Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT218800.17362892467432872Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG218030.17301789052442362Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC217040.17223227518883139Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA215440.17096259383837925No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA160670.1274998141107148No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG158570.12583335733824635Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG154520.12261947641991441Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT151640.12033404998910058Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC151220.12000075863460691Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA149830.11889772296140161No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA148740.11803275254140609No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG146190.116009197889123Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA145300.115302937637934No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG143310.11372377145830918No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCCGTCGGACTGTAGA139340.11057337460749983No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG137550.10915291859668153Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAATGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA136700.10847840037925383No Hit
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC131670.10448683963376994No Hit
ATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGAAC128340.10184431532314145No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA127330.10104282897066853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)