FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824623
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA6100935.6361593378356No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA5587595.161925731732182No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT5085584.698159002858575Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3642113.3646529768288467Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2398622.215892414913665Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2344132.1655534793220976Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1891711.747598969497598Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1734841.6026793727596795No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1502351.3879005301154599No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1389501.2836474766834836Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA866040.8000648151903305No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT845620.7812004168644026Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA798000.7372081226293055No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT759190.701354679973612Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA727070.6716815911279311No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG697290.644170240386201Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG687440.6350706163161526Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT644510.5954110364859819Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG643080.5940899743113456Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG632100.5839464339774235Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA631280.5831889018213383No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA593170.5479821329574249No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT590680.5456818218980929No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC539080.4980127252468746Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT476510.4402093264587599Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG463270.4279779535970907Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA451170.41679973519632046No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG447300.4132245529474791Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG411400.3800594256261858Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA405680.3747751769276399No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC405540.37464584216928387Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG397360.3670889970024822Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG394750.364677827578845Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG366660.33872773213441243Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC357040.3298405865959489Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT337210.3115212418945214Illumina DpnII expression Sequencing Primer (96% over 28bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN329510.3044078301849404No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC322250.2977008991444783Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT310370.28672592107826755Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT309020.28547876447983456Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG306290.2829567366918922Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA303770.28062871104148385No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT299700.27686876485213385Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG299360.27655466615326924Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC299270.2764715223800404Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC285570.26381519245520146No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT271350.25067847628504014Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG270980.2503366629950992Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG267350.2469831974748682Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG266140.24586537563479116No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT261420.24150494663878827Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA257290.23768957126728568Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA251620.23245151355386692No Hit
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT244490.22586467907473542Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA240020.22173520500436827No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT230960.2133653984993288Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT217260.20070906857448984Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA212150.19598834989449518No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT209510.19354946587978167Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT204980.18936456262726192Illumina RNA PCR Primer (100% over 31bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG197280.18225115091768093Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT193420.17868520686586498No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT190680.17615394088089717Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT188660.17428782508176036Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG187830.17352105472864968No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA187120.17286514273984413Illumina RNA PCR Primer (100% over 45bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG184850.17076807201507155Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT179770.16607506792615317Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG177950.16439371606752495Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT176820.16334979980365136Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC174610.16130815826103134Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC172540.15939585147676738No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG172140.15902632359575017Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT170750.15774221420921541Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG167150.15441646328006067Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT166070.15341873800131423Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT159020.14690580909838616Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC155640.14378329850379085Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT151590.14004182870849174Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT149140.13777847043726144Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT148030.13675303056743868Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT147340.13611559497268402Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC146950.13575530528869229Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT144170.13318708651562278Illumina RNA PCR Primer (100% over 31bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC139800.1291499944155099No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG137260.1268034923710507Illumina DpnII expression Sequencing Primer (96% over 26bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT136590.12618453317034692Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA127560.1178424412563837Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT123410.1140085894908303Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG123110.11373144358006741Illumina RNA PCR Primer (100% over 33bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG122780.11342658307822821Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG122460.11313096077341447Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG120160.1110061754575656Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG117490.10853957685177582No Hit
CGCATTATTACTCACGGTACGAGATCGTCGGACTGTAGAACTCTGAACGT117400.10845643307854694Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG117000.10808690519752975Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT115540.10673812843181697Illumina RNA PCR Primer (100% over 35bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT115540.10673812843181697Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT115110.1063408859597235Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC114210.1055094482274348Illumina DpnII expression Sequencing Primer (100% over 26bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT113700.10503830017913787Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG113460.10481658345052756Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT111000.10254398698227181Illumina RNA PCR Primer (100% over 38bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG108630.10035453428724492Illumina RNA PCR Primer (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)