FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001698194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12806930
Sequences flagged as poor quality0
Sequence length126
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA8498156.635587139150443No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT5453334.258108695838893Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4766583.721875578300186Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA4251983.3200618727517055No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3194842.494618148143232No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2323831.814509800553294No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2307291.8015949177515609Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2158981.6857904275263471Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1814391.416725163641872Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1611521.258318738370554No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1580401.2340193941873658Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1555041.214217614994382Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1515061.183000141329733No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1482211.1573499659949729No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1469041.1470664710434115Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG1448561.131075128856018No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1238880.9673512699764892Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1158330.9044556345665979Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1127450.8803436889246681Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1103770.8618536995204941No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG965810.7541307713870538Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA850430.6640389226770194No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG749740.5854174263465171No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC749530.5852534526229158No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA704050.5497414290544259No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT691470.5399186221834584Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG681250.5319385676348664Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT680090.5310328080187836Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA670890.5238491972705402No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT646580.5048672866955625Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT634130.49514598736777665Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG593830.46367864898144995No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG574620.44867895740821573Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG535540.4181642282732864Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT486900.380184790578226Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT480240.37498448105830207Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA458590.3580795709822729No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC457620.35732216854468635Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC413360.322762754227594No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG413160.3226065887765452Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG393910.3075756641131013Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC389910.3044523550921259Illumina DpnII expression Sequencing Primer (100% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN385620.3011026061671298No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC380030.29673778181031674No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA374040.29206062655140613No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT363140.2835496094692483Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT347220.2711188395657664Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG332800.25985931054515016Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT324710.25354241805022754Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT310440.2424000131178979Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA292980.2287667692413404No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA287340.22436290352176516No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC282240.22038068452002157Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG282050.22023232734152526Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT278950.21781176285026932Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA275550.2151569501824403No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG271870.212283505883143Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA269560.2104797949235297No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT268200.20941786985639804Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA258310.20169548830203649No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT254390.19863464546148063Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG245850.19196638070169822No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT243400.1900533539263508Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG236080.1843376984179659Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG234790.18333043125870133Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT233780.18254179573090507Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG219070.17105582680626816No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC213390.16662072799648314No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT213110.16640209636501488Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC211310.16499660730557597Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT210770.1645749605877443Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG207890.16232617809264202Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT206680.161381377113797Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT202400.15803943646135335Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG202240.15791450410051433Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG196300.1532763902043659Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG190700.1489037575750004Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG190410.1486773176709797Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT183230.14307097797832893Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG181930.14205590254651193No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT177240.1383938227194183Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC175210.13680874339127333Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT171080.13358392682711626Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG167670.13092130588673476Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC164760.1286490985739752Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG164650.12856320757589837Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG164270.1282664932189057Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG160230.12511195110772058Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG158070.1234253642363939Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT157350.12286316861261833No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT155250.12122343137660625No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG151700.11845149462049064Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG151190.11805327272031627Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG148920.11628079485091275Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA147390.11508612915038967No Hit
CAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGTG146880.11468790725021531Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC144530.11285296320039229Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG144520.11284515492783985Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT142680.11140843277819118Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT142300.11111171842119853Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG141920.11081500406420586Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT139470.10890197728885846Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG138510.10815238312382436No Hit
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG138410.10807430039829997Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT138120.10784786049427926Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA136050.10623154807592453Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA135410.10573181863256848No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAGA135390.1057162020874636No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC134290.10485729210669537Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA133810.10448249502417832No Hit
TTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTG133730.10442002884375881Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA133560.10428728821036735No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG130220.10167932517785293Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)