FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9402012
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3370713.585094339381826Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2885963.0695132063222212Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2461732.6183012742378975Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1787081.900742096478924Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1630141.7338203780212151Illumina RNA PCR Primer (100% over 45bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1486961.5815338248876942Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1344011.4294919002443307Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1317431.4012213555992057Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG1176001.250796106195142Illumina RNA PCR Primer (100% over 43bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA849830.9038809990882803Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC788640.8387991846851504Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG688340.7321198909339831Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT668030.71051813165097Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC586690.624004734305806Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT578410.6151981086601463Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA564700.6006161234425142No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG546840.5816201893807411Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC492490.5238134135544604Illumina RNA PCR Primer (100% over 39bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG490100.5212714044610877Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG477630.5080082858860423Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT471570.5015628569714653Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA465510.49511742805688824Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG381320.40557276463803704Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT376360.4002972980676902Illumina RNA PCR Primer (100% over 31bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT368760.392213921871191Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC367440.39080996705811477Illumina RNA PCR Primer (100% over 39bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT345440.3674107201735118Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG343750.36561323257192185Illumina RNA PCR Primer (100% over 40bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT329940.35092488714117787Illumina RNA PCR Primer (100% over 38bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG298160.3171236114142377Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG295580.3143795179159524Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT285440.3035945923064127Illumina RNA PCR Primer (100% over 38bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG281390.2992870036753835Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC274860.2923416817591809Illumina RNA PCR Primer (100% over 42bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN272990.2903527457739897No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA261460.27808941320219543No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC261380.27800432503170597Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG252670.26874035046966543Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT249700.26558145214024403Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT244300.2598380006322051Illumina RNA PCR Primer (100% over 31bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT239170.25438172169956813Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG226330.24072507033600896Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT222200.23633239353449026Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG220540.23456681399683385Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT216030.22976996838549027Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG214800.22846173776421472Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT214320.22795120874127794Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT211950.22543047169052752Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT210280.22365425613155993Illumina RNA PCR Primer (100% over 38bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG201520.21433710146296345Illumina RNA PCR Primer (100% over 33bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG197190.20973170423522117Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT196870.20939135155326327Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT195580.20801930480412065Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT190890.20303101080917574Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG185310.1970961109175355Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG184710.1964579496388645Illumina RNA PCR Primer (100% over 34bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC183670.19535180342250147Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG174990.18611973692439449Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT173190.1842052530883815Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT161020.17126121515267156Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG156650.16661327383968452Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT154320.16413508087417883No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT154200.16400744861844466Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT151470.16110381480049166Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT150570.16014657288248516Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG149490.15899788258087738Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC149110.1585937137710524Illumina RNA PCR Primer (100% over 32bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC148210.15763647185304594Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG146650.15597725252850134Illumina RNA PCR Primer (100% over 37bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA143620.15275453807121284Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG142530.15159521174829388Illumina RNA PCR Primer (100% over 34bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG142390.1514463074499373Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG141340.15032952521226306Illumina RNA PCR Primer (100% over 37bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG140520.14945737146474605Illumina RNA PCR Primer (100% over 40bp)
TGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC140080.148989386527054Illumina RNA PCR Primer (100% over 41bp)
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT139020.14786196826806858Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG136840.14554331562223063Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT135210.1438096441485078Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG134700.14326720706163742Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC131850.14023594098795025Illumina RNA PCR Primer (100% over 39bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT130180.13845972542898263Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT127320.13541782333398425Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC126950.1350242905454705Illumina RNA PCR Primer (100% over 39bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC126930.13500301850284813Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT125920.1339287803504186Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT121490.12921702290956447Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT120700.12837677722598098Illumina DpnII expression Sequencing Primer (96% over 28bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT119770.12738762724404096Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT119650.12725999498830676Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCTCCCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG118790.126345297155545Illumina RNA PCR Primer (100% over 36bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT117930.12543059932278325Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC117930.12543059932278325Illumina RNA PCR Primer (100% over 39bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG117720.1252072428752484Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT115920.12329275903923544Illumina RNA PCR Primer (100% over 38bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC115850.12321830689005714Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG115650.12300558646383347Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG115400.12273968593105392Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG110800.11784711612790963Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC110480.11750676344595178Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG109690.1166665177623683Illumina RNA PCR Primer (100% over 40bp)
CGGCCACCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG109440.11640061722958873Illumina RNA PCR Primer (100% over 36bp)
AGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGTA106380.11314599470836667Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG105000.1116782237674234Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGGCCATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT104570.11122087485104253Illumina RNA PCR Primer (100% over 35bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT103650.11024236089041367Illumina RNA PCR Primer (100% over 31bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT102960.10950847541994202Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG102190.10868950177898092Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC101540.10799816039375403Illumina RNA PCR Primer (100% over 39bp)
ACAAACCCTTGTGTCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA101080.10750890341343958Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT98920.10521152281022401Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG95530.10160591158573293Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA95450.10152082341524345Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC95320.10138255513819808Illumina RNA PCR Primer (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)