FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001698198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13032875
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT6945925.329537803439379Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG6363904.88295943911071Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA5571674.27508895773189No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4533623.478603147808906Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4176943.204926004431102Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT3252732.495788534763051Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2904002.228211350143387Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2520161.933694599234628No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1721981.3212587399173243Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1455111.1164919482462619Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1425941.0941100869915503Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1191700.9143799813932075Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG960910.7372970277087749Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC927930.7119917899926148Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT916820.7034671935394148Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT911130.6991013111074879Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG874510.6710031362995502Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT870050.6675810210717128Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG850660.6527032600251287Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG817420.6271985268024132Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG778040.5969826304633474Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG744440.5712016726930934Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC700030.537126305592588Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC674210.5173148672108034No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT653600.5015010118642279Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG651640.4999971226609631Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT640720.49161831138563056Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT640240.49125001198891266No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG636960.48873329944467353Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT633730.4862549514209259Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG617420.47374044483661515Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC609400.4675867757497866Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA545430.41850320823302606No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA488370.37472161744818394No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT467230.35850109818439907Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT463810.3558769649827839Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT439880.3375157054755762Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC419090.3215637378552315Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT418460.3210803448970392Illumina DpnII expression Sequencing Primer (96% over 28bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN398110.3054659850570193No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG386070.2962278085226782Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG374800.2875804456039055Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT374000.28696661327604234Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT372230.285608509250645Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC366850.281480486845765Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG361100.2770685669892483Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT359260.275656752635163Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA356740.27372318080239394No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT354890.2723036935442103Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA349390.2680835962901509No Hit
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT342290.26263583438036503Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT341320.2618915626828309Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT329550.25286055455914375Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA326280.2503515149190029No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG320560.2459626137747811Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG314570.24136654421990544Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC304000.233256284588013Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG303520.2328879851912951Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT303320.2327345271093293Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT277360.21281566807016872Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA273480.20983858128003222Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG268780.20623231635383596Illumina DpnII expression Sequencing Primer (96% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT265060.20337799602927212Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT259980.19948016074734085Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT252580.19380221171460632Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA248700.19082512492446985Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC245510.18837746851711537Illumina DpnII expression Sequencing Primer (100% over 26bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT243160.18657433605401724Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC241740.1854847836720601Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT241390.18521623202861992Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT234590.1799986572417828Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG232820.1786405532163855Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT228080.17500359667379609Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG223270.1713129298025186No Hit
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG222140.17044589163941187Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT222010.1703461438861341Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG214430.16453008257963037Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT210870.16179852872063916Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC204880.1572024591657635Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT204350.15679579524855414Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG204000.156527243605114Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA202910.1556908970584004No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA199620.153166511610063No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA188330.14450380288309372No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT185580.142393754256064Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG183330.14066735083394877Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA182280.13986169590362832No Hit
TCCTCAAGGAGCTTCAGTCTAGGATCGTCGGACTGTAGAACTCTGAACGT180070.13816598409790626Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT179610.13781303050938493Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG178780.13717617946922686Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG176020.13505845793809884Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC174850.13416072815859892No Hit
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT169630.1301554722192916Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG169170.12980251863077027Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG164080.1258970104447407Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC163540.12548267362343304No Hit
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA163140.12517575745950146Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT161790.12413991540623233Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT160910.12346469984558281Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG160440.12310407335296318Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC159170.12212961453248036Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC158530.12163854867018982Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT157470.12082522083577107Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG156640.12018836979561301Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG155380.11922158387922849Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA148420.11388124262681872No Hit
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT148300.11378916777763923Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA146410.11233898890306246No Hit
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG145180.11139522169897279Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC144190.11063560419324209Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA140140.10752807803343467Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGT139600.10711374121212702Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT138690.10641550693918266Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAATTGCGTTTGGACAATCAGATCGTCGGACTGTAGAACTCTGAACGTGT137980.10587073074820405Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT136380.10464306609247769Illumina RNA PCR Primer (100% over 31bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT133510.10244094261626847Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)