FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001698202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14199851
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA166857411.750644425776018No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA12039718.478757981333748No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC4352153.06492652634172No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3610682.542759075429735Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA3303972.3267638512544955No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA3077812.1674945744149006No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2852352.0087182604944234Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA2811631.9800419032565904No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA2342881.6499328056329605No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT2237401.5756503360493008No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1793041.2627174749932235No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1651691.1631741769684767No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1434431.0101725715290955Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA1382760.9737848657707746No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1258270.8861149317693545No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1099550.7743391110230664No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1005320.7079792597823739Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA833590.5870413710679077No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT793230.5586185376170496Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT732050.5155335784861405Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG690700.48641355462110136Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG617050.4345468132024766Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT564710.39768727150728556No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT560320.39459568977167436Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT548220.38607447359834973Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA481820.3393134195563038No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT455940.32108787620377144Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT445620.3138201943104896Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG436910.30768632713117905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN432870.3048412268551269No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT401140.2824959219642516Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG400640.2821438055934531Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG399520.28135506492286433Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC393100.2768338907218111Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA368140.2592562414915481No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC354420.24959416827683617No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG307020.21621353632513468Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC304880.21470647825811692Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT288180.20294579147344577Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG279340.19672037403772757Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA274890.1935865383376206No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC250400.17633987849590818Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC243060.17117081017258562No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG240430.16931867806218528Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT239250.16848768342710074Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAATGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA237690.1673890803502093No Hit
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT236490.1665440010602928Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT232500.16373411242132047Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA230970.16265663632667693No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA229720.1617763453996806No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT213870.1506142564453669Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG195430.1376282046903168Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC193950.13658594023275314Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA192760.13574790327025263No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC190320.13402957538075577Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC187010.1316985650060694Illumina RNA PCR Primer (100% over 39bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT186270.13117743277728758Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT181820.1280435970771806Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG180160.12687457072612943Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC177440.1249590576689854Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT171070.12047309510501202Illumina RNA PCR Primer (100% over 31bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG169500.1193674497007046Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT165960.11687446579545094Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT164670.11596600555879072Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC163770.11533219609135335No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG163190.11492374110122705Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA163150.11489557179156315Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT162850.11468430196908404Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT162280.11428288930637372Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA161000.11338147139712945No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA160290.11288146615059552No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC158430.11157159325122497No Hit
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAAC158150.11137440808357778No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGGACTGTAG155180.10928283684103447No Hit
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT151670.10681097991802871Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT150090.10569829218630533Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT147590.10393771033231265Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT146730.10333207017453915Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT145070.10216304382348802Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)