FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10053557
Sequences flagged as poor quality0
Sequence length126
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA217467421.630891434742946No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA104272510.37170227413044No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2858272.8430435118635127No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2745672.731043351124383No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2203652.1919107834172524Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1932361.9220659911710851No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA1633431.6247284418838028No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1613611.6050140263789223No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA1566061.5577173332781622No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1503311.4953016131504502Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1290781.28390379643742No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1232071.225506554545819No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT950790.9457249807207537Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA943670.9386429101660237No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT918900.9140048641490768Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG876780.872109244519129No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT865540.8609291219018304No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA820020.8156516146474329No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT653050.6495710921020291Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC550830.5478956353457786No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG469780.4672774024158812Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA466820.46433317083694853No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA444540.4421718601684956No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA439510.43716865582997144No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA397060.3949447941658858No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA388200.38613199288570205No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT360510.3585895022030511Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT355660.35376533897405665Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC325980.32424344935827193No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC312460.3107954726869306Illumina DpnII expression Sequencing Primer (100% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN302200.30059012944373814No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT269050.2676167251053533No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT268630.26719896251645064Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG256430.25506395398166043No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA255730.25436768300015605No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC251750.2504088851338884No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA244350.24304830618655668No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG242740.24144688292909666Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG238560.2372891504966849Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG218130.2169679845650649No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC217460.21630155376848215Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC215100.21395412588798174Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAATGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA212440.21130829615826516No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA211090.2099654878367925No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG206690.20558892738162224Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT202490.20141130149259612No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT186390.1853970689179959Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG183250.1822737962295335Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT178500.17754910028361107Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA174100.17317253982844083No Hit
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA173460.17253594921677967No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG172610.17169047731066728No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA171440.17052671009872425No Hit
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA163780.16290751621540514No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA160400.15954552204756983No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG152660.1518467543377931Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAGA137310.13657852638623325No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT135560.13483784893247236Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT134500.13378349573190862No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC131410.13070995668498225No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT128700.12801439331372966Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG127100.12642291678457684No Hit
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA125910.12523925611601944No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG125550.12488117389696005Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA125080.12441367766652142No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT123420.12276252076752538Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG121400.12075328164946994Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGGACTGTAG120380.11973871536213501No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT117390.1167646435982807Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT117010.1163866679226069Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT115790.11517316706912788Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT115740.11512343342759185Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAAGGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA115250.11463604374053879No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA112900.11229856258834559No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT112520.11192058691267179Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT112450.11185095981452137Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT109020.10843923200515003No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC104300.10374437624414921Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC102590.10204348570361714Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA102470.10192412496393068Illumina RNA PCR Primer (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)