FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001698206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10907366
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT6435615.900242093278982Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA5445234.992250191292746No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4323633.9639542672355548Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT3323223.0467667446017677Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2857022.6193491627584513Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC2320832.1277639349408464Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1971291.8073015978376448Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1939181.7778627764026622No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1709101.5669227566031982Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1697421.5562143967663689No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1612201.4780837096692272Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1459971.3385174752547957Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1253311.1490491838267827Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1199411.09963303697703Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1076820.9872410992718131Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1018360.9336442913898737No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG1014330.9299495405215155Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG904520.8292744554459802Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT818710.7506028494872181No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA807840.7406371070705796No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT706220.6474707092436432Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG691920.634360302936566Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC671880.6159873978740604Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC638220.5851275184127863Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT633760.5810385385435861Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACGT631990.579415781958724Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT612180.5612537435710876Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC575130.5272858726845693Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA530060.4859651725265294No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT522220.4787773693483835Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA510670.4681881950234365No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA498690.4572047916976472No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT469420.4303697152914829Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC465790.42704168907507095No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT457200.4191662771745259Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG454280.4164891872153185Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG433130.3970986212436623Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA419550.38464831931008825No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT390460.3579782689972996Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG379530.3479575178828692Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC377870.34643561057729244Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG368000.33738667979051956No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT362310.3321700216165846Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG354710.32520225322960644Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA351360.3221309342695569No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT346730.3178860964232795Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT323750.2968177651689693Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG322130.29533253032858714No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN321700.29493830132774496No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA305080.27970089203937964Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT296140.2715045960683817Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAAC288150.264179271145756No Hit
AGTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAAC282260.25877925064584795Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACCTGTAGAACTCTGAA272960.25025290248809845No Hit
GCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACG269200.246805690759804Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT261560.23980124990763121Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG259380.23780260055452437Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT256860.2354922352472632Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAACTCTGAACG256390.2350613337812264Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACGT245660.2252239449927691Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT245500.22507725513199062Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG235090.2155332460650903Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG226560.20771284286233727Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT218620.20043335852120486Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTGT207410.19015590015041212Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG200190.1835365201827829Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC198930.1823813375291523No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG197930.18146452589928677Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG196070.17975925626773687Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT188460.1727823197644601Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT188410.1727364791829668Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACAAGATCGGATCTACGGGTTGATCCGTCGGACTGTAGAACTCTGAACG182150.16699723838000852Illumina DpnII expression Sequencing Primer (95% over 24bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA179500.16456768756086482No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG176060.16141385555412735Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT174240.15974525838777207Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG174040.15956189606179896Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG173430.159002640967581Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCAGGAGCTCACAATCTAGTGGATCGTCGGACTGTAGAACTCTGAACGT159860.14656150715030558Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA158010.14486540563505435No Hit
CACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG157910.14477372447206777Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG152900.1401804982064414Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACG150660.1381268401555426Illumina DpnII expression Sequencing Primer (96% over 26bp)
TATCAGATATTTTATTGTCATCAAGTAAGATCGTCGGACTGTAGAACTCT150530.13800765464366008Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA149800.13733838215385824No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA149220.13680663140853622No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC143740.13178250367687303Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT141020.12928877604363875Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAG140240.12857366297234363No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG139680.12806024845961894Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG138400.12688672957339103No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC136760.12538315850041157No Hit
TTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTG135790.12449385121944198Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG132720.12167923951575477Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAGAACTCTGAACG130580.1197172626278425Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT128580.11788363936811141Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG127490.116884314691558No Hit
CAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGTG125230.11481232040806186Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC124800.11441809140721967No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC123550.11327207686988774Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT121430.11132843621457279Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG116170.10650600704148003Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG114780.10523163887596694Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT114140.104644879432853Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT113960.1044798533394772Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC111580.1022978416603972Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG111120.10187610831065906Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)