FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001698208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001698208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4980323
Sequences flagged as poor quality0
Sequence length126
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2510645.041118819000294Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1568243.148872071148799Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1144982.2990075141712696Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT799211.60473527520203Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT791821.5898968801822695Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT783421.5730305042464112Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA603651.2120699802000794No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG594691.194079179201831Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT493900.9917027469905064Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA489640.9831490849087499No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG440600.8846815758736933Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT434710.8728550336996216Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT417000.837295091101521Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG391660.7864148570283493Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT385040.7731225464693756Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC380060.7631231950216883Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG360490.7238285548949336Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG329880.6623666778239082Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC315500.6334930485432371Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC305780.6139762421031728Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA295070.5924716127849539No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG254800.5116134033876919Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT223190.44814362441954064Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT222700.44715975248994894Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT221060.44386679337866236Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT218670.4390679078445313Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT215940.4335863356653776Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA208900.41945070630961084No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT204260.41013404150694643Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG200110.40180124863387373Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT186860.37519654849695494Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG168990.3393153415953142Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT157810.3168669983854461Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT152430.30606448617890847Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT151470.3041369003576676Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG151330.3038557940920699Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG150710.3026108949158518Illumina DpnII expression Sequencing Primer (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147950.2970690856797842No Hit
AGTTAGCAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTGA142060.2852425435057124Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT140330.2817688732236845No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA138880.27885741547285187No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT134750.2705647806377217Illumina RNA PCR Primer (100% over 31bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC132330.2657056580466769Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT129500.26002329567781046Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA129280.25958155726044274No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG128830.2586780014067361Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT125570.2521322412221055Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA118940.23881985164416042No Hit
TGGTATTCTCGCACGGACTACAACCACGATCGTCGGACTGTAGAACTCTG118780.2384985873406203Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA116290.2334989116167767No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA115910.2327359088958688Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG115280.23147093070067945Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC112260.2254070669713591No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC110850.2225759252964115Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT110390.22165229042373355Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC110180.2212306310253371Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT110160.22119047298739458Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG109730.22032707517163044Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG106370.21358052479728723Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG105600.21203444033650026No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA104880.2105887509705696No Hit
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG100790.20237643221132445Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTG99930.20064963657979612Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG96940.19464600990738956Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT96890.19454561481253324Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG96190.1931400834845451Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGTTAGCAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTG96080.19291921427586123Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT94510.18976680829737347Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG93200.1871364568121385Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG92210.18514863393398379Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT91520.18376318162496688Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG90550.18181551678475472Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAGCCTTACAGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT89640.17998832605837012Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC89280.17926548137540477Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT86720.17412525251876235Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG86250.17318153862711314Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG80420.16147547056686887Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT80290.16121444332024248Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC79460.15954788474562795Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT78880.1583833016452949Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC78830.1582829065504386No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC77400.15541160683754848No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT76960.15452813000281307Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT76790.15418678668030167Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT76030.15266078123848595Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT75580.15175722538477926Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG73780.14814300196995256Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTGAACGTGT72610.1457937567503152Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTAGCTTACAGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC70870.14230000744931603Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG70680.1419185060888621Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACGT68360.1372601736875299Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA66250.13302350068459415No Hit
AACACTGATTTCAAATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACG65950.13242113011545636Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA64020.12854587945400328No Hit
GGGAGTGAAGACACGGAGCCAGAGATCGTCGGACTGTAGAACTCTGAACG63770.1280439039797218Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT62840.12617655521539467Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT62480.12545371053242932Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT61820.12412849528032618Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG61160.12280328002822306Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT60060.12059458794138453Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG59840.12015284952401682Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTGAACGT58200.11685989041273026Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG57340.11513309478120196Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCAGCACCAGGATATTGTTGATCGTCGGACTGTAGAACTCTGAACGTG56590.11362716835835748Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT54610.1096515226020481Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA54520.10947081143130677No Hit
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT52020.1044510566884919Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACG51910.10423018747980804Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGT51610.10362781691067025Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG51440.10328647358815884Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG50660.10172031010840059Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCACTTTTGTGACTATGCAAGATCGTCGGACTGTAGAACTCTGAACGT50120.10063604308395259Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC50060.10051556897012504Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCATTATTACTCACGGTACGAGATCGTCGGACTGTAGAACTCTGAACGT50040.10047541093218251Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT49990.1003750158373262Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

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