FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001703113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001703113
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences55605
Sequences flagged as poor quality0
Sequence length229-260
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1069419.2320834457333No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA657911.831669813865659No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA35036.299793184066181No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA33726.064202859455085No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA28325.0930671702185055No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA18493.325240535923028No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA17613.1669813865659564No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA16392.9475766567754698No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA12182.1904504990558404No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA8561.5394299073824296No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA7511.3505979678086504No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGCGCA6501.1689596259329198No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA5030.9045949105296287No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4210.7571261577196295No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA3830.6887869795881666No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA3740.6726013847675569No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA3710.6672061864940204No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA3480.62584299973024No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA3240.5826814135419477No Hit
TACGTAGGGGGCAAGCGTTATTCGGAATTACTGGGTGTAAAGGGTGCGTA2570.46218865209963134No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA2550.4585918532506069No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2390.42981746245841196No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA2200.3956478733926805No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA2190.3938494739681683No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2110.3794622785720709No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1940.3488894883553637No Hit
TACGTAGGGGGCACGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1660.29853430446902257No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA1640.2949375056199982No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1550.27875191079938855No Hit
TACGTAGGGTGCACGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1390.24997752000719362No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA1380.2481791205826814No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGATCGTA1320.2373887240356083No Hit
TACGTAGGGGGCTAGCGTTATCCAGATTTACTGGGCGTAAAGGGTGCGTA1290.23199352576207177No Hit
TACGTATGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1210.2176063303659743No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA1170.21041273266792557No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA1140.205017534394389No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1130.2032191349698768No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1090.1960255372718281No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1060.19063033899829152No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1040.18703354014926715No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGCGCGCGCA1000.1798399424512184No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1000.1798399424512184No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGAGGGCGCA1000.1798399424512184No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA980.17624314360219404No Hit
TACGTAGGGGGCAAGCGTTATCCGGCTTTACTGGGTGTAAAGGGAGCGTA930.16725114647963313No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGCGCGTA920.16545274705512095No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA890.16005754878158437No Hit
TACGTATGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA890.16005754878158437No Hit
AACGTAGGTCACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGCA890.16005754878158437No Hit
TACGTATGGTGCACGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA880.1582591493570722No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGCA870.15646074993256No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA850.15286395108353565No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA830.14926715223451129No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA820.1474687528099991No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGCA820.1474687528099991No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA790.14207355453646253No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTACTGGGCGTAAAGGGCTCGTA780.14027515511195035No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA780.14027515511195035No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGCGCGCGCA750.1348799568384138No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA730.13128315798938944No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA690.1240895602913407No Hit
AACGTAGGGTGCAAGCGTTGTTCGGAATTACTGGGTGTAAAGGGAGCGCA680.12229116086682852No Hit
TACGTAGGGTGCAAGCGTTATCCGGACTTATTGGGCGTAAAGGGCTCGTA660.11869436201780414No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA650.11689596259329196No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA640.11509756316877978No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA640.11509756316877978No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA640.11509756316877978No Hit
AACGTAGGTCACACGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA630.1132991637442676No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGCGGGCGCA590.10610556604621886No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA560.1007103677726823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)