FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001703254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001703254
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences85736
Sequences flagged as poor quality0
Sequence length226-264
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA65357.622235700289261No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA61237.141690771671176No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA56676.609825510870579No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA53786.272744238126342No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA46755.452785294392087No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA33883.95166557805356No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA31633.6892320612111598No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA26963.144536717364934No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA24412.8471120649435475No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA22472.6208360548661007No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA20742.419053839693944No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA15761.8382009890827655No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA14191.655080712886069No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA11931.3914808248577029No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA11531.3448259774190539No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA9261.0800597182047214No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA8921.04040309788187No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6370.7429784454604834No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA6170.7196510217411589No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA5430.6333395539796585No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA4980.5808528506111785No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA4200.48987589810581317No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA4170.48637678454791455No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA3920.45721750489875895No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3770.43972193710926566No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA3670.4280582252496034No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA3650.425725482877671No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATAATTGGGCGTAAAGGGCGCGTA3540.4128953998320426No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3300.38490249136885324No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA3050.35574321171969764No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2810.32775030325650834No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA2800.32658393207054215No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGTA2670.3114211066529812No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA2660.31025473546701504No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA2480.289260054119623No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA2420.2822618270038257No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA2270.2647662592143324No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA2240.2612671456564337No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2110.24610432023887283No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGTA2020.23560697956517682No Hit
AACGTAGGTCACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGCA1980.23094149482131193No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1970.2297751236353457No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1930.2251096388914808No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1830.21344592703181864No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGTGCGTA1830.21344592703181864No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGCA1820.2122795558458524No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1800.20994681347391997No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA1700.19828310161425772No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1690.1971167304282915No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1680.1959503592423253No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1660.19361761687039283No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1640.1912848744984604No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1560.1819539050107306No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1480.17262293552300084No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1400.16329196603527107No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGCA1340.15629373891947373No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA1330.15512736773350752No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1320.15396099654754128No Hit
TACGTATGGTGCACGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1310.15279462536157506No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA1300.15162825417560885No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1290.1504618829896426No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA1260.14696276943174394No Hit
AACGTAGGTCACACGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1240.1446300270598115No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA1210.14113091350191284No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1160.13529905757208174No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA1150.13413268638611553No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA1090.1271344592703182No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA1090.1271344592703182No Hit
TACGTATGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1060.12363534571241952No Hit
TACGTAGGGTGCACGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1040.12130260334048708No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1010.11780348978258841No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGATCGTA1000.11663711859662218No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA990.11547074741065597No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA980.11430437622468974No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA970.11313800503872351No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTGTA960.1119716338527573No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA960.1119716338527573No Hit
TACGTAGGGGGCACGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA920.10730614910889243No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA910.1061397779229262No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA900.10497340673695998No Hit
TACGTAGGGAGCACGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA900.10497340673695998No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA900.10497340673695998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)