FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001704412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001704412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3570996
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG198590.5561193571765413No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT172500.48305850804649453No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC153550.4299920806408072No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG130460.36533224904200395No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC98500.27583340894249114No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC97420.2728090426312435No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC94360.26424000474937526No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA86500.2422293388175176No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG71590.20047628168723794No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC66970.18753871468912314No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA64780.18140597189131547No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA63520.17787754452819327No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT59740.1672922624388266No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA58140.1628117197554968No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT57840.16197161800237245No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA56860.15922728560883295No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC53390.14951010866436143No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT51250.1435173828254078No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA50300.14085706060718073No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC45470.1273314223818789No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC44010.12324292718334044No Hit
CTCGACCTTGTCCATGTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCT40380.11307769597053595No Hit
GTCATGGTTGTGCGAGTTTGTGTCAAACTTGCTGTAGGTCTGCTTGAGGA37750.1057128039348126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)