FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001705006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001705006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634655
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG273290.5896663289932045No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC197110.4252959497524627No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG170110.3670391863040507TruSeq Adapter, Index 27 (97% over 38bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG161430.34831071568433897No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT134160.28947138460144284No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT123320.2660823729058581No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC105930.22856070192926983No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC98640.21283137579819855No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC95320.2056679515519494No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA85540.18456605723619127No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA80250.1731520469161135No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG63600.13722704278959275No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC61690.133105916190094No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC61170.13198393407923567No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC60170.12982627617373893No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT59570.12853168143044089No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA59320.1279922669540667No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA58860.1269997443175382No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC58830.12693501458037332No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT57990.12512258193975603No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC55390.11951267138546451No Hit
CTCGACCTTGTCCATGTCCTTCCTGAAGCAGTAGAGCAGCCCGTAGTTCT54110.11675086926642869No Hit
GTCATGGTTGTGCGAGTTTGTGTCAAACTTGCTGTAGGTCTGCTTGAGGA51880.11193929213717095No Hit
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG47530.10255348024826012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)