FastQCFastQC Report
Wed 15 Mar 2023
EGAF00001706827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001706827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95920886
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC9943291.0366136526303562TruSeq Adapter, Index 6 (100% over 50bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3549770.3700726867764753TruSeq Adapter, Index 6 (97% over 49bp)
AGCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG2360800.2461194947678027TruSeq Adapter, Index 6 (98% over 51bp)
AGATGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG1803360.18800493565082374TruSeq Adapter, Index 6 (97% over 48bp)
AGAGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG1702400.17747959500707697TruSeq Adapter, Index 6 (100% over 48bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1123040.11707981930025124TruSeq Adapter, Index 6 (98% over 51bp)
AGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCT1091070.11374686426478588TruSeq Adapter, Index 6 (98% over 51bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)