FastQCFastQC Report
Fri 24 Mar 2023
EGAF00001707670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001707670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130353042
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG19232971.475452333517464RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC18733151.4371087711171329RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC17307621.3277496048001702No Hit
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC13810211.059446698604855No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACT13730941.053365520997968RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG13217401.0139694323359174RNA PCR Primer, Index 1 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA12410900.9520989928259594RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC9804410.7521427846693444RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG9669840.7418192818238949Illumina Small RNA Adapter 2 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA8390770.6436957566360438RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG7029120.539237128044929RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA6610690.5071373785047533RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA6574770.5043817849682404RNA PCR Primer, Index 1 (100% over 28bp)
CTCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA6514160.4997321044490853RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG6382960.4896671302845391Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC6000530.4603291114602451RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCC5747640.44092872032859803RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4397330.3373400369129859RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACTC4336410.33266657482377743RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC4122640.3162672644033884RNA PCR Primer, Index 1 (100% over 24bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG4001750.30699321922997397RNA PCR Primer, Index 1 (100% over 29bp)
GAAGTGCTTCGATTTTGGGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3826770.2935696736559474RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3796320.2912337097587642RNA PCR Primer, Index 1 (100% over 29bp)
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3514550.2696177968750434RNA PCR Primer, Index 1 (100% over 32bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG3195860.24516957571270181RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTCC3170950.24325861148679598RNA PCR Primer, Index 1 (100% over 27bp)
AAAGTGCTGCGACATTTGAGCGTTGGAATTCTCGGGTGCCAAGGAACTCCA3016470.23140771812597974RNA PCR Primer, Index 1 (100% over 28bp)
TAAGTGCTTCCATGTTTTGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3014800.2312796045066597RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTC2952820.22652482479081693RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAAC2940960.22561498794941817RNA PCR Primer, Index 1 (100% over 24bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG2903740.22275966524816504Illumina Small RNA Adapter 2 (100% over 21bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2896640.22221499057919952RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG2803060.2150360250127496No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA2736380.20992068600900007No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCTGGAATTCTCGGGTGCCAAGGA2652120.20345670183899509RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC2566680.1969021942733028RNA PCR Primer, Index 1 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTTCTGTGGAATTCTCGGGTGCCAAGG2554530.1959701101566928Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGA2551650.19574917169942227RNA PCR Primer, Index 1 (100% over 22bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG2320310.17800198326019886Illumina Small RNA Adapter 2 (100% over 21bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGGTGGAATTCTCGGGTGCCAAGGAAC2259230.17331624681225316RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2252920.1728321767895528No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2239100.17177197905362268Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA2204850.16914449913642982No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2151920.16508398783666284RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAACCCAGAGGTCGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2142110.16433141621658512RNA PCR Primer, Index 1 (100% over 30bp)
TAAGTGCTTCCATGTTTTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2137630.16398773417194207RNA PCR Primer, Index 1 (100% over 30bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2070920.1588700937259293RNA PCR Primer, Index 1 (100% over 31bp)
TAAGTGCTTCCATGTTTTAGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA2070150.15881102337450628RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA2048030.1571140932790813No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1983780.15218517109865376RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1830340.14041406106962967RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGGTGCCAAGGAACTC1809710.1388314359399453RNA PCR Primer, Index 1 (100% over 26bp)
CGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1780530.13659289976523908RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1747760.13407895766636577RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1735370.13312846201164988RNA PCR Primer, Index 1 (100% over 29bp)
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1694030.12995707457291256RNA PCR Primer, Index 1 (100% over 29bp)
CCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1674850.12848568581928452RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1584650.12156601608115905RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1569270.12038614334754076RNA PCR Primer, Index 1 (100% over 27bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCAG1564700.120035556976108RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG1549900.11890017879291225Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGTGGAATTCTCGGGTGCCA1540690.11819363601809922No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCCA1518490.1164905687433056RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT1479230.11347874796815251RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1472680.1129762664073463RNA PCR Primer, Index 1 (100% over 29bp)
TAAGTGCTTCCATGTTTGAGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1471630.11289571592813308RNA PCR Primer, Index 1 (100% over 28bp)
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1448370.11111133102670516RNA PCR Primer, Index 1 (100% over 31bp)
TAAACGTGGATGTACTTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1435630.11013398521225151RNA PCR Primer, Index 1 (100% over 30bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA1418090.10878840863568033RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA1413890.10846620671882748No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC1347300.10335777204186765RNA PCR Primer, Index 1 (100% over 26bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG1337590.1026128718960007RNA PCR Primer, Index 1 (100% over 29bp)
AATGGATAAGGCACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1334990.10241341356652038RNA PCR Primer, Index 1 (100% over 35bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT1313050.10073029212467478RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)