FastQCFastQC Report
Mon 6 Mar 2023
EGAF00001707673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001707673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152214468
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC26172981.7194804372998234RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA24494121.6091847458284978RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG22046981.4484155343235834RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACT18748521.2317173424013808RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA11332610.7445159549485139RNA PCR Primer, Index 1 (100% over 28bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC11247820.738945525204608No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC9743380.6401086656230339RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC8619140.5662497207558482No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACTC6098750.40066822031661276RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG4993220.32803846215196836RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC4908590.3224785438924242RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTCC4771280.3134577194068044RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA4430100.29104329294111514RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG4235220.27824030498861646Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA4230320.2779183907800407RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC3973160.26102380753976684RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC3970520.2608503680477995RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA3941470.2589418766683861RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC3794240.24926934015234345RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3703750.24332443877805363RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3306310.21721391162369663RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG3295050.21647416591174498RNA PCR Primer, Index 1 (100% over 29bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGGTGGAATTCTCGGGTGCCAAGGAAC3218820.21146610058118787RNA PCR Primer, Index 1 (100% over 24bp)
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3197610.21007267193549564RNA PCR Primer, Index 1 (100% over 31bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3050670.20041918748485854RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAACCCAGAGGTCGATGTGGAATTCTCGGGTGCCAAGGAACTCCAGT2929000.1924258605955907RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2538670.16678243752755487RNA PCR Primer, Index 1 (100% over 29bp)
AATGGATAAGGCACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA2409450.15829309996997132RNA PCR Primer, Index 7 (97% over 35bp)
TAAGTGCTTCCATGTTTTGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG2352450.15454838366613086RNA PCR Primer, Index 1 (100% over 29bp)
GAGTTCTGGGCTGTAGTGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2329780.15305903772563853RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTC2277440.14962046840383136RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2268340.1490226277307621Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2183950.14347847669776043RNA PCR Primer, Index 1 (100% over 29bp)
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTCCA2121350.13936585844126198RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAAC2090320.13732728744287304RNA PCR Primer, Index 1 (100% over 24bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGTGGAATTCTCGGGTGCCAAGGAACT2089270.13725830582674967RNA PCR Primer, Index 1 (100% over 25bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCAG2070380.1360172937043015RNA PCR Primer, Index 1 (100% over 29bp)
CGGCGTCCGGTGAGCTCTCGCTGGTGGAATTCTCGGGTGCCAAGGAACTCC2024510.13300378253136882RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2021680.13281786065172202RNA PCR Primer, Index 1 (100% over 28bp)
CGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1968130.1292997982294298RNA PCR Primer, Index 1 (100% over 30bp)
TAAACGTGGATGTACTTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1960550.1288018166577963RNA PCR Primer, Index 1 (100% over 30bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT1944920.12777497602921686RNA PCR Primer, Index 1 (100% over 25bp)
GTGTAATGGTTAGCACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1930210.126808576435717RNA PCR Primer, Index 1 (100% over 31bp)
AGTTCTGGGCTGTAGTGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1920760.1261877418906066RNA PCR Primer, Index 1 (100% over 30bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC1897230.12464189672167036RNA PCR Primer, Index 1 (100% over 27bp)
GAGTTCTGGGCTGTAGTGCGCTATGGAATTCTCGGGTGCCAAGGAACTCCA1779470.11690544423149053RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1735820.11403777990407589RNA PCR Primer, Index 1 (100% over 29bp)
CTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1715250.1126863971958303RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1673200.10992384771203222RNA PCR Primer, Index 1 (100% over 27bp)
ACAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1672400.10987129029022392RNA PCR Primer, Index 1 (100% over 30bp)
CGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG1645110.10807842523878873RNA PCR Primer, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA1603500.1053447823369852No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1597120.10492563689806411RNA PCR Primer, Index 1 (100% over 27bp)
CGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA1591270.10454131075109102RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA1584390.10408931692353976RNA PCR Primer, Index 1 (100% over 28bp)
AATTAAAACAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGAA1542010.10130508750324574RNA PCR Primer, Index 1 (100% over 23bp)
CAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1532700.1006934505069518RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1532600.10068688082922578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)