FastQCFastQC Report
Fri 24 May 2024
EGAF00001707673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001707673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152214468
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC26216681.7223513864661013RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC24646811.6192159867483817RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA22081071.450655137460389RNA PCR Primer, Index 1 (100% over 28bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC18781721.2338984754064246RNA PCR Primer, Index 1 (100% over 24bp)
CTCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCC11608300.7626278994714221RNA PCR Primer, Index 1 (100% over 27bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTG11317980.7435548110971948No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC9759520.6411690116080161RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC8639930.5676155567550911No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAACT6107780.40126146221527376RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA5000570.328521333464832RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAA4959550.32582645166161206RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACTC4778990.3139642415594817RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG4513470.2965204332613113Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC4266510.28029595714909306RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG4247340.279036549929012No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT3978720.26138908162133445RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC3976950.2612727983255836RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3959650.2601362440789794RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC3800140.2496569511381796RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3710270.24375278176579115RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA3311920.21758247054412724RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA3300840.2168545502520825RNA PCR Primer, Index 1 (100% over 28bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGGTGGAATTCTCGGGTGCCAAGGAA3238850.21278200702971284RNA PCR Primer, Index 1 (100% over 23bp)
CCTCCCCGGAGGGGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3204140.21050167189100577RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA3054940.20069971272376028RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAACCCAGAGGTCGATGTGGAATTCTCGGGTGCCAAGGAACTCCAG2937920.19301187584875307RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2544320.1671536243190759RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATAAGGCACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2413840.15858150882214428RNA PCR Primer, Index 3 (100% over 34bp)
TAAGTGCTTCCATGTTTTGGTGTGGAATTCTCGGGTGCCAAGGAACTCCA2355500.15474875883677497RNA PCR Primer, Index 1 (100% over 28bp)
GAGTTCTGGGCTGTAGTGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2333110.15327780799391555RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAGGAACT2281090.14986026164083166RNA PCR Primer, Index 1 (100% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG2274770.1494450580085462No Hit
CCAAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCA2188390.14377017038879641RNA PCR Primer, Index 1 (100% over 28bp)
TTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTCC2131600.14003925040818066RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCGACTCTGGACGCGAGCCGGGCCTGGAATTCTCGGGTGCCAAGGAA2117960.13914314636634936RNA PCR Primer, Index 1 (100% over 23bp)
CTAAGGGCTGGGTCGGTCGGGCTGGGTGGAATTCTCGGGTGCCAAGGAAC2092680.13748233183720748RNA PCR Primer, Index 1 (100% over 24bp)
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA2073460.13621963977826337RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTGCCAAGGAACTCC2048760.13459692937993253RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGTCCGGTGAGCTCTCGCTGGTGGAATTCTCGGGTGCCAAGGAACTC2027630.13320875647642116RNA PCR Primer, Index 1 (100% over 26bp)
CGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1977850.1299383709044005RNA PCR Primer, Index 1 (100% over 29bp)
TAAACGTGGATGTACTTGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1966930.12922096209671738RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAAC1949140.1280522164292556RNA PCR Primer, Index 1 (100% over 24bp)
GTGTAATGGTTAGCACTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1934090.1270634799314872RNA PCR Primer, Index 1 (100% over 30bp)
AGTTCTGGGCTGTAGTGCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1924240.12641636667547265RNA PCR Primer, Index 1 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTC1900280.12484227189231446RNA PCR Primer, Index 1 (100% over 26bp)
GAGTTCTGGGCTGTAGTGCGCTATGGAATTCTCGGGTGCCAAGGAACTCC1807520.11874823883364358RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1738600.11422041694485965RNA PCR Primer, Index 1 (100% over 28bp)
CTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1717510.11283487191243871RNA PCR Primer, Index 1 (100% over 30bp)
ACAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1678420.11026678488933127RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1676130.11011633926940505RNA PCR Primer, Index 1 (100% over 26bp)
CGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1647300.10822230118098893RNA PCR Primer, Index 10 (100% over 35bp)
CGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAACTCC1618720.10634468728688787RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1614070.10603919727262721No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCC1609340.1057284515161857RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1599660.10509250671230544RNA PCR Primer, Index 1 (100% over 26bp)
AATTAAAACAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGA1546290.10158626970992009RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1538460.10107186394397148No Hit
CAAAGCATCGCGAAGGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1534740.10082747193256295RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)