FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001708076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001708076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4841242
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG235340.4861149267068244No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG150520.3109119519329957No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC148310.3063470076480374No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC143460.29632891724892085No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT133710.2761894571682225No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT90380.18668763098395No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC83150.17175344673949372No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC78950.16307798701242368No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG63760.13170174099952037TruSeq Adapter, Index 6 (97% over 35bp)
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC59850.12362530111074803No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC58910.12168365060040377No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA58410.12065085777575259No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA58300.12042364335432933No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT53140.109765221403929No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC53070.10962063040847782No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG50650.10462191313716604No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC49650.10255632748786365No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG49180.10158550223269153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)