FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001720376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35108505
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3703161.054775758751334No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG3355960.9558823424694387TruSeq Adapter, Index 6 (97% over 35bp)
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2911190.8291979393597079No Hit
CATAATGGCGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC501640.14288275732618064No Hit
CATAATGGCGAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC445940.12701765569339965No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG397490.11321758075429302TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)