FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001720379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29819493
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTCAAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3112661.0438339779955346No Hit
TCACTTCAAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2097030.703241332775175No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC1257010.4215396955273519Illumina Single End PCR Primer 1 (96% over 33bp)
TCACTTCAAGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC920170.30858002850685623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)