FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001720405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41931260
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTAAATTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5043331.2027613765958856No Hit
CCGTAAATTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3896880.9293496069519495No Hit
CCGTAAATTACGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1122210.26763087968260435No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC755420.18015676132794484Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)