FastQCFastQC Report
Sat 13 May 2023
EGAF00001720406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28638157
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG5162931.8028150345009981TruSeq Adapter, Index 6 (97% over 35bp)
GTCTTTGATTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2431780.8491398381536912No Hit
GTCTTTGATTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1508390.5267063798833145No Hit
GTCTTTGATTAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC796110.27798925747910386No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG529420.18486524813730157TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)