FastQCFastQC Report
Sun 14 May 2023
EGAF00001720418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36263336
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAATGGAGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT401571211.07375228798586No Hit
AGAAATGGAGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA22286166.145645287570895No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG20731125.716826493844913TruSeq Adapter, Index 1 (97% over 35bp)
AGAAATGGAGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC9399932.5921305199278963No Hit
AGAAATGGAGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC7016311.9348219921079517No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATG2128620.586989569850937TruSeq Adapter, Index 1 (97% over 35bp)
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA583610.16093665513840205No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC555610.15321535779278553No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTATG472470.1302886198886942TruSeq Adapter, Index 1 (97% over 35bp)
TGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGC379640.10468976158178056No Hit
TCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAG376620.10385696451093193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)