FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001720424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27830591
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG4135751.4860446190309073TruSeq Adapter, Index 27 (97% over 38bp)
CAGCGCTGGTTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1581760.5683530040738265No Hit
CAGCGCTGGTTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1032820.371109618189567No Hit
CAGCGCTGGTTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC627540.22548568947026673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)