FastQCFastQC Report
Thu 23 Mar 2023
EGAF00001720428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46723438
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTTAATCGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5179921.1086341719973603No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG4379820.9373924923932181TruSeq Adapter, Index 7 (97% over 35bp)
CAGCTTAATCGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3167180.6778567964112572No Hit
CAGCTTAATCGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1529390.3273282244341694No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTATG727010.15559856704037917TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)