FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001720432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25823138
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG5569652.1568447645673428TruSeq Adapter, Index 6 (97% over 35bp)
GTTGACGAATAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1198620.46416512199253246No Hit
GTTGACGAATATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA597730.23147070661977642No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG547670.21208499137478953TruSeq Adapter, Index 6 (97% over 35bp)
GTTGACGAATAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC510150.19755538618118373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)