FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001720434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31554524
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGCAAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT17332045.492727445357756No Hit
CTCCACGCAAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA13551414.2946013066145445No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG8995022.8506276944630824TruSeq Adapter, Index 13 (97% over 37bp)
CTCCACGCAAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2717190.8611094878186087No Hit
CTCCACGCAAGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1525830.48355348348781935No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAACTCGTATG1048580.3323073420470548TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)