FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001720435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31554524
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCACGCAAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16431135.207218464141623No Hit
CTCCACGCAAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA12953844.105224341206985No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC4520151.4324887296667825Illumina Single End PCR Primer 1 (96% over 31bp)
CTCCACGCAAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2576490.8165200020130235No Hit
CTCCACGCAAGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1358960.4306704167047489No Hit
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTTAAGATTAGTGTAGATCTC499710.15836397975770447Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATTTC329580.1044477806098422Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)