FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001720441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23698304
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC4005951.690395228283003Illumina Single End PCR Primer 1 (96% over 31bp)
AGCCATATGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1468050.6194747100889583No Hit
AGCCATATGAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA926320.3908802925306385No Hit
AGCCATATGAGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC540580.22810915076454416No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCTC356710.15052132000669752Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)