FastQCFastQC Report
Wed 20 Sep 2023
EGAF00001720447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37149417
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGGAAACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9848262.6509864205944336No Hit
CCTGGGAAACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA7991342.151134700175779No Hit
CCTGGGAAACTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1843440.4962231305002714No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC860300.23157833136385422Illumina Single End PCR Primer 1 (96% over 33bp)
CCTGGGAAACTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC495730.13344220179821395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)