FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001720449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37119614
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT4922071.3260024740558995No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2988970.8052265845221344No Hit
GAATCCCCCTAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC1500240.4041636855383248No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC642990.17322109006844738Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)