FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001720450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29249663
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAAAACCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3264081.1159376434525075No Hit
GGTATAAAACCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2343580.801233162925672No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG772970.2642662925723281TruSeq Adapter, Index 7 (97% over 34bp)
GGTATAAAACCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC718320.24558231662361374No Hit
GGTATAAAACCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC446440.15263081834481307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)