FastQCFastQC Report
Sat 13 May 2023
EGAF00001720454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001720454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29412833
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG1434590.48774288420296TruSeq Adapter, Index 27 (97% over 38bp)
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1129780.38411124831123883No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA559730.19030128787662176No Hit
GAATCCCCCTAGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC522130.1775177522002046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)