FastQCFastQC Report
Sat 11 May 2024
EGAF00001721566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41386694
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATAT9241452.2329519724382916No Hit
TATATATATATATATATATATATATATATATATATATATATA5973861.4434252709336968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGACAACA3112310.7520073963868678TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA699940.16912198882085147No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT546500.13204727103836802Clontech SMART CDS Primer II A (100% over 26bp)
TATATATATATATATATATATATATATATATATATATATATT477740.11543323561915818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)