FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001721568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44535356
Sequences flagged as poor quality0
Sequence length42
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATAT1082263724.301224851553897No Hit
TATATATATATATATATATATATATATATATATATATATATA662051614.865752953675726No Hit
TATATATATATATATATATATATATATATATATATATATATT5317461.1939861893099046No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACACACA2334100.5241004472940556TruSeq Adapter, Index 4 (97% over 37bp)
ATATATATATATATATATATATATATATATATATATATATAG756970.16997057349221592No Hit
TATATATATATATATATATATATATATATATATATATATTTA578030.1297912606783698No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA542330.12177515769717885Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATATATATATATATATAGAT513200.11523428711336674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495830.11133401515865282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)