FastQCFastQC Report
Wed 14 Sep 2022
EGAF00001721572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35756388
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATAT11172093.124501837266113No Hit
TATATATATATATATATATATATATATATATATATATATATA8641312.41671781836577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCA5149281.440100717108227TruSeq Adapter, Index 7 (97% over 38bp)
TATATATATATATATATATATATATATATATATATATATATT650550.18193951805199116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA592250.16563473916884447No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT436640.12211524273648669Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)