FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001721574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36957189
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACA397326910.75100435804249TruSeq Adapter, Index 6 (97% over 40bp)
ATATATATATATATATATATATATATATATATATATATATAT1892600.5121060478923329No Hit
TATATATATATATATATATATATATATATATATATATATATA1383000.37421677281786775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA736040.19916016881045798No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACA726400.196551745318076TruSeq Adapter, Index 6 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT543390.14703228646529368Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)