FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001721682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4052204
Sequences flagged as poor quality0
Sequence length30-51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGA127670.3150631113339802Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGA74710.18436880275524134Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG56940.1405161240648299No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGAC54980.13567925010685544Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGA50510.12464821613127079Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA45500.11228457402440745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)