FastQCFastQC Report
Sat 13 May 2023
EGAF00001721727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001721727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81665325
Sequences flagged as poor quality0
Sequence length30-51
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCG3369720.4126255543585971No Hit
ACCTATTTATCTATTTATTAACTTTAAATCCAAATTATAAAACCAATTA2760410.33801494085770184No Hit
AATAATTCCATTCCATTAAATTCCATTCCATTCCATTCCCCTACACTTA2726930.3339152816694233No Hit
AATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCA2559100.31336433180177753No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACCA1753780.2147520994987775No Hit
AAAAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTCG1479490.1811650170987503No Hit
AACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG1316680.1612287712073637No Hit
AATAATTCCATTCCATTACATTCCGTTCCATTACATTCCCCTACACTCG1259340.1542074313669847No Hit
ACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCTTATTAACCA1087650.1331838206729723No Hit
AACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT913190.11182102073309572No Hit
ACTACGTTTATTTATTTATTTTTTTAATTATTTTACTTTTTTTTAATTT877100.10740176445755896No Hit
AAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA834360.10216820908996566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)